Chromatography Resolution Calculator (Rs from Two Peaks)

This chromatography resolution calculator computes peak resolution (Rs) using retention times and peak widths at half height for two adjacent peaks. Suitable for GC and HPLC method optimization and system suitability evaluation.


Chromatography Resolution Calculator Interface


Reference Formula

\[ R_s = \frac{2\left(t_{R2} – t_{R1}\right)} {1.699\left(w_{1/2,1} + w_{1/2,2}\right)} \]

Before You Go

Remember that this uses peak width at half height (w½). Ensure both peaks are measured consistently using the same integration method.

For baseline separation, a resolution (Rs) of 1.5 or greater is generally recommended. Use this calculator during method development to optimize selectivity, efficiency, and separation performance.

The article explaining how this calculator works will be published soon at GC Hardware.

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If you frequently work with gas chromatography, don’t forget to explore our full collection of Free GC Calculators.

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