This chromatography resolution calculator computes peak resolution (Rs) using retention times and peak widths for two adjacent peaks. Suitable for GC and HPLC method optimization and system suitability evaluation.
Chromatography Resolution Calculator Interface
Reference Formula
For half-height width:
\[ R_s = \frac{2 \times {(t_{R2} – t_{R1})}} {1.699 \times {(w_{1/2,1} + w_{1/2,2})}} \]For baseline width:
\[ R_s = \frac{2 \times {(t_{R2} – t_{R1})}} {w_1 + w_2} \]Before You Go
For baseline separation, a resolution (Rs) of 1.5 or greater is generally recommended. Use this calculator during method development to optimize selectivity, efficiency, and separation performance.
The article explaining how this calculator works is available at GC Peak Resolution Calculation: What It Really Means in Practice in Gas Chromatography Columns series.
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